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KMID : 0545120050150020447
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Journal of Microbiology and Biotechnology
2005 Volume.15 No. 2 p.447 ~ p.450
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Increased Production of Exoinulinase in Saccharomyces cerevisiae by Expressing the Kluyveromyces marxianus INU1 Gene Under the Control of the INU1 Promoter
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Kim HE
Qin R/Chae KS
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Abstract
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Aspergillus niger inuE gene and Kluyveromyces marxianus INU1 gene coding for exoinulinase were expressed in Saccharomyces cerevisiae under the control of K. marxianus INU1 promoter. Recombinant S. cerevisiae expressing K. marxianus exoinulinase produced maximum 85 U/ml into culture medium which was 9- to 14-fold higher than the activity produced by any other strain reported so far. In addition K. marxianus INU1 promoter produced 20-fold higher activity than S. cerevisiae glyceraldehydes phosphate dehydrogenase (GPD) promoter in S. cerevisiae.
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